Effects of Yeast Concentration and Microalgal Species on Improving the Performance of Microalgal-Microbial Fuel Cells (MMFCs)

Hadiyanto Hadiyanto, Marcelinus Christwardana, Tifany Minasheila, Yosafat Hans Wijaya


This research aimed to determine the effects of various operating conditions such as yeast concentration (816 mg/L), anolyte pH (36) and type of microalga used (Spirulina or Chlorella) on the performance of microalgal-microbial fuel cells (MMFCs). MMFCs featuring a salt bridge, tofu wastewater in the anode chamber and microalga in the cathode chamber were constructed, and their performance was analyzed in terms of power density production, biomass growth, and chemical oxygen demand (COD) removal. Result showed that Spirulina produces a higher power density (maximum 0.98 mW/m2) than Chlorella (maximum 0.39 mW/m2). The COD removal rate in the microalgae coupled to microbial fuel cells operated at pH =3 was higher than those of MMFCs operated at other pH, and Chlorella showed higher carbon utilization from COD than Spriulina. A yeast concentration of 16 mg/L resulted in the best operating conditions.


bioelectricity; microalgae-microbial fuel cell; power density; S. cerevisiae; tofu waste treatment

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Hadiyanto H., Christwardana M., and da Costa C., 2019. Electrogenic and biomass production capabilities of a microalgae–microbial fuel cell (MMFC) system using tapioca wastewater and Spirulina platensis for COD reduction. Energy Sources, Part A: Recovery, Utilization and Environmental Effects: 1–12. doi.org/10.1080/15567036.2019.1668085.

Rashid N., Cui Y., Rehman M.S., and Han J.I., 2013. Enhanced electricity generation by using algae biomass and activated sludge in microbial fuel cell. Science of the Total Environment 456–467: 91–94.

Saba B., Christy A.D., Yu Z., and Co A.C., 2017. Sustainable power generation from bacterio-algal microbial fuel cells (MFCs): An overview. Renewable and Sustainable Energy Reviews 73: 75–84. doi.org/10.1016/j.rser.2017.01.115.

Uggetti E. and J. Puigagut. 2016. Photosynthetic membrane-less microbial fuel cells to enhance microalgal biomass concentration. Bioresource Technology 218: 1016–1020.

Maity J.P., Bundschuh J., Chen C.Y., and Bhattachary P., 2014. Microalgae for third generation biofuel production, mitigation of greenhouse gas emissions and wastewater treatment: Present and future perspectives – A mini review. Energy 78: 104–113.

Hadiyanto H., Soetrinanto D., Silviana S., Mahdi M.Z., and Titisari Y.N., 2017. Evaluation of growth and biomass productivity of marine microalga Nannochloropsis sp. cultured in palm oil mill effluent (POME). Philippine Journal of Science 146 (4): 355–360.

Nur M.M.A. and H. Hadiyanto. 2015. Enhancement of Chlorella vulgaris biomass cultivated in pome medium as biofuel feedstock under mixotrophic conditions. Journal of Engineering and Technological Sciences 47 (5): 487–497. doi: 10.5614/j.eng.technol.sci.2015.47.5.2.

Ichsan I., Hadiyanto H., and Hendroko R., 2014. Integrated biogas-microalgae from waste waters as the potential biorefinery sources in Indonesia. Energy Procedia 47: 143–148.

Gajda I., Greenman J., Melhuish C., and Ieropoulos I., 2013. Photosynthetic cathodes for microbial fuel cells. Int. J. Hydrogen Energy 38: 1559–1564.

Park J.B.K., Craggs R.J., and Shilton A.N., 2011. Recycling algae to improve species control and harvest efficiency from a high rate algal pond. Water Res 45 (20): 6637–6649.

Powell E.E., Mapiour M.L., Evitts R.W., and Hill G.A., 2009. Growth kinetics of Chlorella vulgaris and its use as a cathodic half cell. Bioresource Technology 100(1): 269–274.

Harnisch F., Warmbier R., Schneider R., and Schroder U., 2009. Modeling the ion transfer and polarization of ion exchange membranes in bioelectrochemical systems. Bioelectrochemistry 75:136–141.

Schaetzle O., Barrière F., and Baronian K., 2008. Bacteria and yeasts as catalysts in microbial fuel cells: Electron transfer from micro-organisms to electrodes for green electricity. Energy Environ. Sci. 1: 607–620. doi: 10.1039/B810642H.

Spiegel, C. 2007. Designing and building fuel cells. The McFraw-Hill, New York, USA.

Fleury D., 2017. A modular photosynthetic microbial fuel cell with interchangeable algae solar compartments. Biorxiv doi: https://doi.org/10.1101/166793

Margarites A.C., Volpato N., Araújo E., Cardoso L.G., Bertolin T.E., Colla L.M., and Costa J.A.V., 2016. Spirulina platensis is more efficient than Chlorella homosphaera in carbohydrate productivity. Environmental Technology 38(17): 2209–2216. doi:10.1080/09593330.2016.1254685.

Juang D., Lee C.H., and Hsueh S.C., 2012. Comparison of electrogenic capabilities of microbial fuel cell with different light power on algae grown cathode. Bioresources Technology 123:23–9.

Karuppiah T., Pugazhendi A., Subramanian S., Jamal M.T., and Jeyakumar R.B., 2018. Deriving electricity from dye processing wastewater using single chamber microbial fuel cell with carbon brush anode and planitum nano coated air cathode. 3 Biotech 8(10): 437.

Singh S. and D.S. Songera. 2012. A review on microbial fuel cell using organic waste as feed. CIBTech Journal of Biotechnology 2(1): 17–27.

Hadiyanto H., Nur M.M.A., and Hartanto G.D., 2012. Cultivation of chlorella sp. as biofuel sources in palm oil mill effluent (POME). International Journal of Renewable Energy Development 1(2):45–49.

Lal D., 2010. Microbes to generate electricity. Indian Journal of Microbiology 53(1): 120–122.

Logan B.E., 2008. Microbial Fuel Cells. New Jersey: John Wiley & Sons.

Cui Y., Lai B., and Tang X., 2019. Microbial fuel cell-based biosensors. Biosensors 9(3):92.

Doherty L., Zhao Y., Zhao X., Hu Y., Hao X., Xu L., and Liu R., 2015. A review of a recently emerged technology: Constructed wetland – Microbial fuel cells. Water Research 85: 38–45.

Motto S.A., Christwardana M., and Hadiyanto H., 2018. Potency of yeast – Microalgae spirulina collaboration in microalgae-microbial fuel cells. In Proceedings of the IOP Conference Series: Earth and Environmental Science, Indonesia, 209 (012022).

Ling J., Xu Y., Lu C., He P., Chen J., Zheng L., Talawar M.P., Xie G., Du Q., 2019. Accelerated lipid production from distillery wastewater by Rhodosporidium toruloides using an open-bubble-column reactor under non-aseptic conditions. International Biodeterioration & Biodegradation 143: 104720.

Pena A., Sanchez N.S., Alfarez H., Calahorra M., and Ramirez J., 2015. Effects of high medium pH on growth, metabolism and transport in Saccharomyces cerevisiae. FEMS Yeast Research 15(2): 1–13.

Gunawardena A., Fernando S., and To F., 2008. Performance of a yeast-mediated biological fuel cell. International Journal of Molecular Sciences 9(10): 1893–1907.

Lee D.J., Chang J.S., and Lai J.Y., 2015. Microalgae-micobial fuel cell: A mini review. Bioresource Technology 198: 891–895.

Winfield J., Ieropoulos I., Greenman J., and Dennis J., 2011. The overshoot phenomenon as a function of internal resistance in microbial fuel cells. Bioelectrochemistry 81: 22–27.